deep-sequencing data Search Results


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SciClone Inc deep-sequencing data
Deep Sequencing Data, supplied by SciClone Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Solexa deep sequencing data
PIK3R1 is the direct target of gga-miR-16-5p. ( a ) Alignments of PIK3R1 3′-UTR derived from several species. The highlighted U to A <t>sequence</t> is the conserved target region. ( b ) Sequence alignments of gga-miR-16-5p. Position 2988–2995 in the 3′-UTR of PIK3R1, which is highlighted, was predicted to be the target site of it. The seed sequence in gga-miR-16-5p is also highlighted. ( c ) The recombinant plasmid and gga-miR-16-5p mimics were cotransfected into DF-1 cells. The cells were assayed firefly and Renilla luciferase by dual-luciferase assay transfected 24 h later. All data from the triplicate experiments carried out independently were adopted as mean value ± SD. (Different lowercase letters between groups mean p < 0.05.)
Deep Sequencing Data, supplied by Solexa, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Biotechnology Information deep sequencing data
PIK3R1 is the direct target of gga-miR-16-5p. ( a ) Alignments of PIK3R1 3′-UTR derived from several species. The highlighted U to A <t>sequence</t> is the conserved target region. ( b ) Sequence alignments of gga-miR-16-5p. Position 2988–2995 in the 3′-UTR of PIK3R1, which is highlighted, was predicted to be the target site of it. The seed sequence in gga-miR-16-5p is also highlighted. ( c ) The recombinant plasmid and gga-miR-16-5p mimics were cotransfected into DF-1 cells. The cells were assayed firefly and Renilla luciferase by dual-luciferase assay transfected 24 h later. All data from the triplicate experiments carried out independently were adopted as mean value ± SD. (Different lowercase letters between groups mean p < 0.05.)
Deep Sequencing Data, supplied by Biotechnology Information, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Temasek Laboratories deep sequencing data set
PIK3R1 is the direct target of gga-miR-16-5p. ( a ) Alignments of PIK3R1 3′-UTR derived from several species. The highlighted U to A <t>sequence</t> is the conserved target region. ( b ) Sequence alignments of gga-miR-16-5p. Position 2988–2995 in the 3′-UTR of PIK3R1, which is highlighted, was predicted to be the target site of it. The seed sequence in gga-miR-16-5p is also highlighted. ( c ) The recombinant plasmid and gga-miR-16-5p mimics were cotransfected into DF-1 cells. The cells were assayed firefly and Renilla luciferase by dual-luciferase assay transfected 24 h later. All data from the triplicate experiments carried out independently were adopted as mean value ± SD. (Different lowercase letters between groups mean p < 0.05.)
Deep Sequencing Data Set, supplied by Temasek Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cold Spring Harbor Laboratory Meetings encode deep sequencing data
PIK3R1 is the direct target of gga-miR-16-5p. ( a ) Alignments of PIK3R1 3′-UTR derived from several species. The highlighted U to A <t>sequence</t> is the conserved target region. ( b ) Sequence alignments of gga-miR-16-5p. Position 2988–2995 in the 3′-UTR of PIK3R1, which is highlighted, was predicted to be the target site of it. The seed sequence in gga-miR-16-5p is also highlighted. ( c ) The recombinant plasmid and gga-miR-16-5p mimics were cotransfected into DF-1 cells. The cells were assayed firefly and Renilla luciferase by dual-luciferase assay transfected 24 h later. All data from the triplicate experiments carried out independently were adopted as mean value ± SD. (Different lowercase letters between groups mean p < 0.05.)
Encode Deep Sequencing Data, supplied by Cold Spring Harbor Laboratory Meetings, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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encode deep sequencing data - by Bioz Stars, 2026-05
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PIK3R1 is the direct target of gga-miR-16-5p. ( a ) Alignments of PIK3R1 3′-UTR derived from several species. The highlighted U to A sequence is the conserved target region. ( b ) Sequence alignments of gga-miR-16-5p. Position 2988–2995 in the 3′-UTR of PIK3R1, which is highlighted, was predicted to be the target site of it. The seed sequence in gga-miR-16-5p is also highlighted. ( c ) The recombinant plasmid and gga-miR-16-5p mimics were cotransfected into DF-1 cells. The cells were assayed firefly and Renilla luciferase by dual-luciferase assay transfected 24 h later. All data from the triplicate experiments carried out independently were adopted as mean value ± SD. (Different lowercase letters between groups mean p < 0.05.)

Journal: International Journal of Molecular Sciences

Article Title: Upregulated gga-miR-16-5p Inhibits the Proliferation Cycle and Promotes the Apoptosis of MG -Infected DF-1 Cells by Repressing PIK3R1-Mediated the PI3K/Akt/NF-κB Pathway to Exert Anti-Inflammatory Effect

doi: 10.3390/ijms20051036

Figure Lengend Snippet: PIK3R1 is the direct target of gga-miR-16-5p. ( a ) Alignments of PIK3R1 3′-UTR derived from several species. The highlighted U to A sequence is the conserved target region. ( b ) Sequence alignments of gga-miR-16-5p. Position 2988–2995 in the 3′-UTR of PIK3R1, which is highlighted, was predicted to be the target site of it. The seed sequence in gga-miR-16-5p is also highlighted. ( c ) The recombinant plasmid and gga-miR-16-5p mimics were cotransfected into DF-1 cells. The cells were assayed firefly and Renilla luciferase by dual-luciferase assay transfected 24 h later. All data from the triplicate experiments carried out independently were adopted as mean value ± SD. (Different lowercase letters between groups mean p < 0.05.)

Article Snippet: Our pilot study presented that gga-miR-16-5p expression was significantly upregulated in embryonic lungs infected by MG according to Solexa deep sequencing data [ ]; therefore, we speculate that gga-miRr-16-5p may play a role in MG -HS infection.

Techniques: Derivative Assay, Sequencing, Recombinant, Plasmid Preparation, Luciferase, Transfection